小麦玉米转基因植株的抗性鉴定.doc

1目录中文摘要1英文摘要21引言31.1植物PGIP概况31.2转基因植株的鉴定方法的研究进展31.2.1标记基因的使用31.2.1.1选择标记基因31.2.1.2报告基因41.2.2分子检测41.2.2.1分子标记51.2.2.2分子杂交51.2.3免疫技术61.2.3.1酶联免疫法(ELISA)61.2.3.2免疫荧光技术61.3.小麦赤霉病、玉米纹枯病真菌病害人工接种方法的研究进展71.3.1小麦赤霉病人工接种方法71.3.2玉米纹枯病人工接种方法71.4本研究的目的和意义82材料与方法82.1实验材料82.1.1目的基因82.1.2植物材料82.1.3菌株和载体92.2实验方法92.2.1转基因植株的鉴定92.2.1.1转化株的PCR检测92.2.1.1.1转基因植株DNA的微量提取（CTAB法）92.2.1.1.2转基因植株的PCR鉴定92.2.2转化株的PPT涂叶检测102.2.3真菌病害抗性鉴定102.2.3.1小麦赤霉病抗性鉴定102.2.3.2玉米纹枯病抗性鉴定103.结果与分析113.1转化株的PCR检测113.2转化株的PPT涂叶检测123.3转化株的真菌抗病性鉴定133.3.1小麦赤霉病的抗性鉴定133.3.2玉米纹枯病的抗性鉴定44.讨论155.结论16参考文献17致谢18ContentsAbstract1Englishabstract21Introduction31.1PlantPGIPProfile31.2Progressinidentificationoftransgenicplants31.2.1Theuseofmarkergenes31.2.1.1Selectablemarkergene31.2.1.2Reportergene41.2.2Moleculardetection41.2.2.1Molecularmarkers51.2.2.2Molecularhybridization51.2.3Immunoassaytechnology61.2.3.1ELISA.61.2.3.2Immunofluorescencetechnique61.3.wheatscabblotchfungaldiseasesofcornsheathblightartificialinoculationmethod71.3.1WheatFusariumheadblightartificialinoculationmethods71.3.2Maizesheathblightartificialinoculationmethods71.4Thepurposeandsignificanceofthisstudy82Materialsandmethods82.1Experimentalmaterials82.1.1Thetargetgene82.1.2Plantmaterial82.1.3Strainsandvector92.2Experimentalmethods92.2.1Identificationoftransgenicplants92.2.1.1PCRdetectionoftransformingstrains92.2.1.1.1SwitchtotransgenicplantsDNAExtraction（CTAB)92.2.1.1.2PCRidentificationoftransgenicplants92.2.2TransformingstrainsPPTcoatingsolutiondetection102.2.3Identificationoffungaldiseaseresistance102.2.3.1Wheatscabresistanceidentification102.2.3.2Identificationofcornsheathblightresistance103ResultsandAnalysis113.1PCRidentificationoftransgenicplants113.2TransformingstrainsPPTcoatingsolutiondetection123.3Identificationoffungaldiseaseresistance133.3.1Wheatscabresistanceidentification133.3.2Identificationofcornsheathblightresistance144Discuss155Conclusion16References16Acknowledgement1717小麦玉米转基因植株的抗性鉴定摘要：多聚半乳糖醛酸酶抑制蛋白（polygalacturonaseinhibitingprotein，PGIP）是一种富含亮氨酸