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基于高通量测序的过敏性紫癜患儿肠道菌群研究.docx

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基于高通量测序的过敏性紫癜患儿肠道菌群研究 Title:GutMicrobiotaCompositioninChildrenwithAllergicPurpura:insightsfromHigh-throughputSequencing Abstract: Allergicpurpura,alsoknownasHenoch-Schönleinpurpura(HSP),isasystemicautoimmunevasculitischaracterizedbyskinrash,jointpain,abdominalpain,andkidneyinvolvement.AlthoughtheexactetiologyofHSPremainsunclear,evidencesuggeststhatdysbiosisoftheintestinalmicrobiotamayplayaroleindiseasedevelopment.Inthisstudy,weaimedtoinvestigatethecompositionofgutmicrobiotainchildrenwithHSPusinghigh-throughputsequencingtechniques.OurfindingsmayprovidevaluableinsightsintothepathogenesisofHSPandpotentialtherapeuticstrategiestargetingthegutmicrobiota. Introduction: Allergicpurpuraisacommonchildhooddiseasewithvariousclinicalpresentations.WhileseveralfactorshavebeenimplicatedinthedevelopmentofHSP,theroleofgutmicrobiotahasgainedsignificantattentioninrecentyears.Theimmensecomplexityofthegutmicrobialcommunityanditsimpactonhosthealthanddiseasemakeitapromisingareaofresearch.High-throughputsequencingplatformshaverevolutionizedourabilitytostudythegutmicrobiotacompositionwithgreatdepthandaccuracy.Inthisstudy,weexploredthepotentialrelationshipbetweengutmicrobiotaandallergicpurpurabyutilizinghigh-throughputsequencingtechnologies. Methods: Werecruitedacohortofchildrendiagnosedwithallergicpurpuraandage-matchedhealthycontrols.Fecalsampleswerecollectedfrombothgroups,andDNAwasextractedforsubsequentsequencinganalysis.High-throughputsequencingofthe16SrRNAgenewasperformedtodeterminethecompositionanddiversityofthegutmicrobiota.Bioinformaticstoolswereemployedtoanalyzethesequencingdata,includingalphaandbetadiversityindices,taxonomicprofiling,andidentificationofdifferentiallyabundantbacteria. Results: Preliminaryanalysisofthesequencingdatarevealedsignificantalterationsinthegutmicrobiotacompositionofchildrenwithallergicpurpuracomparedtohealthycontrols.Alphadiversityindicesindicatedreducedmicrobialdiversityinthediseasegroup,suggestingdysbiosis.Betadiversityanalysisdemonstrateddistinctclustering